Use Viruses to Deliver Genetic Material into Bacteria
- Discuss applications for viral-induced genetic engineering
- Materials for 8 lab groups
- Time Required: ~120 minutes
Demonstrate transduction using a mutant E. coli, which is incapable of producing beta-galactosidase for lactose breakdown, and a lambda phage carrying the lac+ phenotype. Students will observe and differentiate
… View More Use Viruses to Deliver Genetic Material into Bacteria
- Discuss applications for viral-induced genetic engineering
- Materials for 8 lab groups
- Time Required: ~120 minutes
Demonstrate transduction using a mutant E. coli, which is incapable of producing beta-galactosidase for lactose breakdown, and a lambda phage carrying the lac+ phenotype. Students will observe and differentiate between plaques and colonies to discover how this relates the lytic and lysogenic phases of transduction, and then will use their results to calculate the incidence of transduction for each plate. It includes enough materials for eight setups, a teacher’s guide, and student copymaster.
Ordering information: Coupon is included for redemption of perishable materials. Redeem by mail, phone, fax, or email. Activity includes 2 Tubes of tryptic soy broth; 2 Bottles; Luria agar; 1 Sleeve; Petri plates; 2 Cotton swabs; 2 Vials of N,N-dimethylformamide; 50 Disposable pipettes; 16 Capped vial; 20 Bacti-spreaders; 1 Tube of Sterile water; 1 Redemption coupon for: 1 Culture of E. coli host strain; 1 Tube of lambda phage gt11; 2 Vials of X-gal; and 2 Vials of IPTG.
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