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Taste SNP analysis
Blotting
Chemical formula
Kit 345 refill components
Taste SNP analysis
Blotting
Chemical formula
Kit 345 refill components

Exploring the Genetics of Taste: SNP Analysis of the PTC Gene using PCR
345-FB 345
470330-708EA 152.99 USD
470330-708 470330-706
Exploring the Genetics of Taste: SNP Analysis of the PTC Gene using PCR
Educational Materials Biology Educational Materials PCR Learning Activities
Let your students isolate, amplify and predict their ability to taste PTC paper.

  • Students isolate and analyze their own DNA
  • Total time (with stop-points) approximately 4 hours
  • Perishable component refills available
The objective of this experiment is to identify the presence of the single nucleotide polymorphism (SNP) in an amplified segment of the PTC gene that links detection of the characteristic taste of PTC paper.

This is a set of five modules that starts with (I) extraction of DNA from buccal cells (II) amplifying the segment that contains the polymorphic nucleotide (III) digestion of the amplified fragment with the restriction enzyme that recognizes the SNP (IV) analysis by gel electrophoresis (V) tasting the PTC paper to confirm the results obtained.

Time Required: Extraction - 30 min; PCR Setup - 10 min; PCR - 2 hours; DNA Digest - 1 hr; Electrophoresis - 30 min.; Staining - 5 min.

Ordering information: Kit will ship immediately upon ordering. Please call to place your order if you would like to specify a future date for shipment.

Caution: Some components require Freezer storage upon receipt.
   
 

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