Methyl Violet 2B

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Disposal

This disposal method is published for your convenience. You MUST have checked with your federal, state, and local regulations before using this method and these methods are only applicable for small laboratory sized quantities.

The disposal of this chemical does not need pretreatment and it is not necessary for any chemical reaction to be conducted before it is disposed into a landfill or down the school drain. HOWEVER, there are numerous conditions that should be met which are outlined below before this can be discharged.

If the material is in the solid form, it can be buried in a landfill if:
 a. Local regulations have been confirmed that this material can be disposed of in a landfill.
 b. Chemical is packed into a cardboard box with an appropriate amount of packing material.
 c. Cardboard box is sealed with durable tape.

If the material is in an aqueous form, it can be poured down the drain with excess water if:
 a. Local and State regulations have been checked and you have verified that this material is suitable for this type of disposal
 b. The school drain is connected to the sanitary sewer system.
 c. The quantity that you are disposing of is minimal. A good rule of thumb is less than 250 mL.
 d. Combinations of chemicals are not going down the drain at the same time.
 e. A twenty-fold excess of water is used when washing the chemical down the drain.

A good resource to contact is the local wastewater treatment facility as they can give you guidance and be a resource on drain disposal.

Solution Prep

To prepare a Methyl Violet 2B solution, use a 0.04% aqueous solution. Add 0.04 g of methyl violet 2B to 75 ml of DI water. Dilute to a final volume of 100 ml with DI water. To prepare a Methyl Violet 2B stain, prepare a 0.7% sodium chloride solution (0.7 g of NaCl in 100 ml of DI water) with 1 ml of 1 M acetic acid. Add 0.05 g of methyl violet 2B. Stir and filter. Use a 0.9% sodium chloride solution if staining human blood cells.

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